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. 2017 Nov 30;3(2):e000604. doi: 10.1136/rmdopen-2017-000604

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Suramin treatment increases proteoglycan accumulation during chondrogenic differentiation. (A) Alcian Blue staining of ATDC5 micromass cultures at day 1 (D1) and at day 21 (D21) showing increased proteoglycan content in the presence of suramin compared with controls, as quantified by (B) colourimetry at day 21 (*P<0.05, unpaired t-test), and by (C) di-methylmethylene blue (DMMB) assay determining total sulfated glycosaminoglycan content normalised to DNA content at different time points (P<0.001 for interaction between time and treatment, two-way analysis of variance; *P<0.001 between groups at days 14 and 21, Sidak multiple comparisons test). Data shown are representative of three independent biological replicates. Error bars indicate mean±SEM of three technical replicates per experiment.