Basolateral PAR-2 stimulation increases CBF in primary mouse septal ALIs. A, B) 2FLI and trypsin, but not AY-NH2, stimulated CBF in wild-type primary mouse nasal septal ALI cultures. Peak CBF increases were 1.89 ± 0.20 (2FLI), 1.16 ± 0.04 (AY-NH2), 1.81 ± 0.14 (trypsin). C, D) Basolateral 2FLI (C) and trypsin (D) stimulated CBF increases in wild-type (left red traces), but not PAR-2-knockout (PAR-2 KO), ALIs. E) Bar graph summarizing results from C and D. N.s. not significant. *P < 0.05, **P < 0.01.