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. 2017 Sep 6;32(1):183–194. doi: 10.1096/fj.201700166RR

Figure 4.

Figure 4.

Overexpression of adtrp1 increases the expression levels of scl and runx1 and the number of HSCs. A, B) Overexpression of adtrp1 increased the expression level of scl at 12 hpf. C–F) Overexpression of adtrp1 increased the expression level of runx1 (C, D), a marker for HSCs at 30 hpf, and the number of erythrocytes with o-dianisidine staining (E, F) at 48 hpf. G) The number of cmyb+kdrl+ cells in Tg(cmyb:GFP/kdrl:mCherry) embryos was increased in embryos that were injected with adtrp1 mRNA compared with control GFP mRNA. White arrowheads mark cmyb+kdrl+ cells in the ventral wall of the dorsal aorta region at 48 hpf. Embryos were injected with 200 pg of control gfp mRNA group or 200 pg of adtrp1 mRNA, grown to appropriate embryonic stages, and used for whole-mount in situ hybridization with the antisense probe for each marker. Embryos at 12 hpf are shown in a dorsal view with the anterior to the top. Embryos before 48 hpf are shown in a lateral view with the anterior to the left. Black arrowheads mark the differences between 2 groups.