Figure 5.
FFAR2-FFAR3 heteromerization enables p38 phosphorylation in HEK293 and monocytes. This effect was abolished by FFAR2 antagonism and Gαq inhibition. A, B) HEK293 cell lines that stably express FFAR3, FFAR2, or both (i.e., control-, FFAR3-, FFAR2- and FFAR2-FFAR3-HEK293) were stimulated with 10 mM acetate or 10 mM propionate for 6 min (A) or indicated times (B). Western blots are representative of 3 independent experiments. Optical density (OD) measurements of 3 independent experiments are shown (means ± sd). C) Monocytes were stimulated with 10 mM acetate for 3 min. Western blot displays samples from 3 different donors. Corresponding OD is shown as means ± sd; n = 3. Vehicle control has been arbitrarily assigned value of 1. Two-tailed Welch’s t test was used to determine statistical significance. *P < 0.05, **P < 0.01, ***P < 0.001.