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. 2017 Aug 31;32(1):52–62. doi: 10.1096/fj.201700375R

Figure 6.

Figure 6.

Bzb injections promote trabecular osteoblast survival and DNA repair and suppress marrow adiposity in mice after SARRP radiation. A) TUNEL staining revealed that 2 wk of Bzb injections reversed the radiation-induced osteoblast apoptosis (green, indicated by white arrowheads) in the trabecular bone of Col2/Tomato mice. Dashed lines: trabecular bone perimeters. B) Quantification of the percentage of TUNEL+ cells among Tomato+ osteoblasts within the metaphysis. C) Immunofluorescent staining of γH2AX foci (white, indicated by arrows) in trabecular osteoblasts at 2 wk after radiation and Bzb injections. Inset: a magnified image of an osteoblast with γH2AX foci (indicated by a yellow arrow in lower magnification image). D) Quantification of the number of γ-H2AX foci per Tomato+ osteoblast on the BS. E) Immunofluorescent staining of perilipin (white outline) to detect adipocytes (yellow asterisks) in bone marrow at 2 wk after radiation and Bzb injections. Endomucin staining shows bone marrow blood vessels (green outline). F) Quantification of the percentage of perilipin+ adipocytes among Tomato+ bone marrow (BM) mesenchymal cells (n = 3 femurs/group). NR, nonirradiated; R, irradiated; veh, vehicle. aP < 0.05, bP < 0.01 R vs. NR; $P < 0.01 Bzb vs. veh.