Figure 3.

Primary neutrophils of mice are specifically activated by PSMs via mFpr2. PSMs induce calcium influx in primary neutrophils of mice. A, B) Calcium influx is induced by PSMα2, PSMα3, and fMIFL (A) or culture filtrates of USA300 WT or its isogenic PSM mutant (0.09%; culture filtrates diluted in RPMI 1640) (B) in neutrophils isolated from WT or mFpr2^−/− mice. C) Migration is induced by PSMα3 or fMIFL in neutrophils of WT or mFpr2^−/− mice. Data represent means ± sem of at least 3 independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significantly different vs. mFpr2^−/− neutrophils, as calculated by Student’s t test.