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Hoxb8 neutrophils expressing mFpr2 are activated by PSMs. A, B) Calcium influx is induced by PSMα2, PSMα3, δ-toxin, or fMIFL in WT Hoxb8 (A) or mFpr2−/− Hoxb8 (B). C) 5-Carboxytetramethylrhodamine-labeled PSMα3 binds specifically to mFpr2-expressing Hoxb8 cells. Data represent means ± sem of at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. corresponding medium control (A, B) or vs. mFpr2−/− Hoxb8 cells (C), as calculated by Student’s t test.
