Figure 5.

Hoxb8 neutrophils expressing mFpr2 up-regulate CD11b and migrate toward and release MIP2 upon PSM stimulation. A, B) PSMs (α2, α3, δ-toxin) and fMIFL induce CD11b up-regulation in WT Hoxb8 cells (A) or mFpr2^−/− Hoxb8 cells (B). C) Migration is induced by 2.5 µM PSMα2, 1 µM PSMα3, or 10 nM fMIFL in WT Hoxb8 or mFpr2^−/− Hoxb8 cells. D) MIP2 secretion is induced by PSMs and fMIFL in WT and mFpr2^−/− Hoxb8 cells. APC, allophycocyanin. Data represent means ± sem of at least 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. corresponding medium control (A, B) or vs. mFpr2^−/− neutrophils (C, D), as calculated by Student’s t test.