Figure 1.

A representative total ion current chromatogram for the cumulative instrument response for all unlabeled and [¹³C] isotopologs measured for glutamic acid, alanine, proline, and glutamine present in mosquito whole-body extracts. Each peak is labeled with the name and structure (with numbered carbon atoms) for each corresponding amino acid, and Table 1 lists the number of transitions monitored in the acquisition method, the retention time of interest, and the dMRM (a specific time-shared MRM mode utilized by Agilent triple-quadrupole mass spectrometers) acquisition window specified for each amino acid and its corresponding isotopologs. Mosquitoes were fed on a BM, BM supplemented with glucose, or BM supplemented with [1,2-¹³C₂] glucose. Data are representative of 3 independent experiments.