Figure 4.

Unlabeled and [¹³C] isotopologs of amino acids and amino acid derivatives present in mosquito whole-body extracts. Measurements were made using an Orbitrap HRAM-LC/MS instrument. Plots are of the normalized mass isotopomer distributions against the degree of [¹³C] labeling for metabolites that exhibited enrichment of [¹³C] labeling greater than the isotopically labeled background. Each column corresponds to a single metabolite isotopolog series, and each individual data plot corresponds to an individual time point during the time course studied. Three groups of mosquitoes were fed on 3 different diets: BM, BM supplemented with glucose, and BM supplemented with [1,2-¹³C₂]glucose, as indicated in Materials and Methods.