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Number of transitions monitored in the acquisition method, the retention time of interest, and the dMRM acquisition window specified for each amino acid and its corresponding isotopologs
| Amino acid | Transitions monitored (n) | Retention time (min) | dMRM window (min) |
|---|---|---|---|
| Glutamic acid | 21 | 6.6 | 6.1–7.1 |
| Alanine | 10 | 7.1 | 6.6–7.6 |
| Proline | 18 | 8.2 | 7.7–8.7 |
| Glutamine | 21 | 8.8 | 8.2–9.2 |
