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. Author manuscript; available in PMC: 2017 Dec 15.
Published in final edited form as: Acta Trop. 2017 Jun 9;173:102–108. doi: 10.1016/j.actatropica.2017.06.004

Fig. 3.

Fig. 3

Topical treatment of nanoliposomal Amphotericin B leads to increased Th1, Th2 cytokines and T cell proliferation in L. mexicana infected 129SVE mice. Draining lymph nodes were collected from control and SinaAmphoLeish treated 129SVE mice infected with L. mexicana at week 15 and re-stimulated with 20 ug/ml L. mexicana antigen for 72 h. Cytokines produced in the culture supernatants were determined by ELISA. Concentrations of (A) IFN-γ, (B) IL-4 and (C) IL-10 produced by L. mexicana re-stimulated lymph node cells of infected 129SVE mice treated with SinaAmphoLeish or vehicle control. (D) T cell proliferation and (E) nitric oxide production were determined by alamar blue assay and Greiss assay respectively. Data represents the means ± SE from one of the two similar experiments (n = 3 to 5 mice per group). * p < 0.05 (Students unpaired t-test).