a, Timeline of myocardial ischaemia reperfusion experimental
protocol. DOX, doxycycline administration. b, Mid-ventricular
cross-sections of hearts 24 h after reperfusion and EBD perfusion and TTC
staining (non-blue area indicates the area at risk (AAR); red area, viable
myocardium; white area, infarct). c, Per cent AAR of the left
ventricle and per cent infarct of the AAR. n = 11 mice
per group. d, TUNEL+ cardiomyocytes post
ischaemia reperfusion. n = 5 mice per group.
e, f, Invasive haemodynamics post ischaemia
reperfusion. e, dP/dt maximum
(contractility). f, dP/dt minimum
(relaxation). n =13–16 mice per group.
g, DHE staining in live myocardium 24 h post ischaemia
reperfusion. n = 6 tTA and n
=5 NCLX-Tg. h, Timeline of myocardial infarction (MI)
experimental protocol. Echo, left ventricular echocardiography. i,
Left-ventricular end-diastolic dimension (LVEDD). j,
Left-ventricular end-systolic dimension (LVESD). k, Per cent
fractional shortening (FS). n =19 tTA,
n =16 NCLX-Tg. l, Heart weight to body
weight ratio. n = 6, sham tTA, n
= 7 sham NCLX-Tg, n =17 tTA, n
=17 NCLX-Tg. m, Images of Masson’s
trichrome-stained left ventricular sections at the border zone 4 weeks post
myocardial infarction. Scale bars, 400 μm. n, Per cent
fibrosis in peri-infarct and remote regions of the left ventricle 4 weeks post
infarction. n = 3 mice per group. o, Per
cent infarct of AAR 24 h after LCA ligation and scar of left ventricle 4 weeks
post infarction. n = 5 mice per group. p,
DHE staining in live myocardium 4 weeks post infarction. n
= 8–9 mice per group. *P <0.05,
**P <0.01,
***P <0.001;
#P < 0.05 versus sham control (l,
n).