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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Biotechniques. 2017 Dec 1;63(6):275–280. doi: 10.2144/000114620

Figure 2. Detergent-free protein lysates were successfully detected on CAM ELISA array.

Figure 2

(A) Representative Stains-All Assay absorbance spectra comparing SDS positive (blue) and negative (purple) controls to column-processed ASC storage protein lysates (shades of red). Lack of 440–450 nm peak indicates successful removal of SDS detergent. (B) Quantified protein expression of CAMs expressed in ASC lysates comparing fresh and fixed/stored samples. Data represented as arithmetic mean ± s.d. from one independent experiment run in biological triplicate (technical quadruplicate). Within each POI, one-way ANOVAs were performed to determine significance.