Figure 2.

Cannabinoid acids induce PPARγ transcriptional activity and PPARγ degradation. (A–C) HEK‐293T cells were transiently transfected with PPARγ‐GAL4 plus GAL4‐luc and incubated with increasing concentrations of the indicated neutral and cannabinoid acids for 6 h (n = 5). (D) Transfected HEK‐293T cells were stimulated with two phytoextracts derived from the Cannabis variety MONIEK before and after decarboxylation (n = 5). (E) STHdh ^Q7/Q7 cells were treated with Δ⁹‐THCA, Δ⁹‐THC and rosiglitazone (RGZ) for 6 h, and the steady‐state levels of endogenous PPARγ and β‐actin detected by Western blots (n = 5). (F) STHdh ^Q7/Q7 cells were transiently transfected with PPARγ‐GAL4 plus GAL4‐luc and incubated with increasing concentrations of Δ⁹‐THCA or Δ⁹‐THC for 6 h (n = 5). ^* P < 0.05, significantly different from untreated cells.