Figure 4.

Δ⁹‐THCA increases mitochondrial biogenesis in N2a cells. (A) The cells were treated with Δ⁹‐THCA, Δ⁹‐THC and rosiglitazone (RGZ) for 72 h, and mitochondria stained with the Mitotracker Green dye (n = 5). (B) Quantification of fluorescence intensity (100% = control untreated cells). (C) Δ⁹‐THCA up‐regulated the expression of PGC‐1α. N2a cells were stimulated with rosiglitazone, Δ⁹‐THCA or Δ⁹‐THC, and the levels of PGC‐1α mRNA were analysed by qPCR (n = 5). ^* P < 0.05, significantly different from control.