Figure 5.

(A) Percentage of cells in pre G0/G1 of LHCN-M2 cells after pretreatment with sumac extracts and addition of H₂O₂. Cells were pre-treated with 1 and 3 μg/mL of the EtOAc fraction, 1 μg/mL of CE, 1 μ g/mL of VitC for 48 h, and incubated with 75 μM of H₂O₂. The histogram shows the percentage of cells of the total population at pre G0-G1. Values are means of three independent experiments. The significance (^∗∗∗p ≤ 0.001) of cells in pre G0/G1 in treated cells/ +H₂O₂ with respect to untreated cells/−H₂O₂. (B) EtOAc fraction of sumac restores human myoblast adhesion under oxidative stress conditions. Cells were pre-treated with 1 and 3 μg/mL of the EtOAc fraction, 1 μg/mL of CE, 1 μg/mL of VitC for XX hours, and incubated with 75 μM of H₂O₂. The results of count were expressed as the percentage of treated cells normalized to untreated control cells. The untreated cells and the solvent treated cells were used as a negative control. Mean values (% of control) with S.D. are indicated. This experiment was performed in duplicates and repeated three times. The significance (^∗∗∗p < 0.001) of cell viability in treated cells/ +H₂O₂ with respect to untreated cells/−H₂O₂.