Table 1. Primers designed for real-time PCR experiment (retrieved from Primer-Blast®).
Gene of interest | Primer sequence |
---|---|
myoD | F: 5′-ACAACGGACGACTTCTATGAC-3′ R: 5′-TGCTCTTCGGGTTTCAGGA-3′ |
myf5 | F: 5′-CATGCCCGAATGTAACAGTC-3′ R: 5′-CCCAGGTTGCTCTGAGG-3′ |
myogenin | F: 5′-ACCCCGCTTCTATGATGG-3′ R: 5′-ACACCGACTTCCTCTTACACA-3′ |
GPx3 | F: 5′-CGGGGACAAGAGAAGTCG-3′ R: 5′-CCCAGAATGACCAGACCG-3′ |
SOD 2 | F: 5′-GGAGATGTTACAGCCCAGATAG-3′ R: 5′-CAAAGGAACCAAAGTCACG-3′ |
Catalase | F: 5′-CTGACTACGGGAGCCAC R: 5′-TGATGAGCGGGTTACACG |
GAPDH | F: 5′-TGGTGCTCAGTGTAGCCCAG-3′ R: 5′-GGACCTGACCTGCCGTCTAG-3′ |