Table 1.

Promoter and terminator part characterization

	Genetic context
	Isolation (Cytometry)b		Circuit (RNA‐seq)c
Promotersa	1st	2nd	1st	2nd
PTac‐PTet1	54	85	8 ± 2	17 ± 5
PBAD1‐PTet2	49	85	242 ± 118	283 ± 237
PBAD2	49		26 ± 29
PBM3R1‐PAmtR	10	75	9 ± 4	55 ± 32
PSrpR‐PLitR	41	85	37 ± 20	14 ± 4
PPhlF	80		323 ± 25
Terminators
L3S2P55	418		24 ± 6
L3S2P24	212		295 ± 176
L3S2P11	384		110 ± 89
ECK120029600	374		380 ± 98
ECK120033737	391		870 ± 344
L3S2P21	505		187 ± 127

^aThe strength of the left promoter is 1^st; right is 2^nd.

^bPreviously reported promoter strengths (in au/s) based on a fluorescent reporter (Nielsen et al, 2016), converted to au/s as described in text. Previously reported termination strengths (Chen et al, 2013).

^cAverage and standard deviations are calculated from three replicates performed on different days. For promoters, all states where the promoter is predicted to be on are included and the units are au/s. For double promoters, separate strengths for each promoter are calculated as described in the text. Median terminator strengths are calculated for states where the upstream gene is on. For terminators L3S2P24 and L3S2P11 in one replicate, the data for input state −/−/+ were excluded due to a mapping bias (Appendix Fig S1).