Table 2.
Sensor and gate response function parameters
Genetic context | ||||||||
---|---|---|---|---|---|---|---|---|
Isolation (Cytometry)a | Circuit (RNA‐seq)b | |||||||
Sensor | δJ off | δJ on | δJ off | δJ on | ||||
PTac | 0.1 | 54 | 0.0 ± 0.0 | 9 ± 0.9 | ||||
PTet1 | 0.0 | 85 | 0.0 ± 0.0 | 16.0 ± 4.8 | ||||
PTet2 | 0.0 | 85 | 0.8 ± 0.6 | 274 ± 165 | ||||
PBAD1 | 0.2 | 49 | 0.8 ± 0.6 | 165 ± 114 | ||||
PBAD2 | 0.2 | 49 | 0.0 ± 0.0 | 13.0 ± 9.2 |
Gate |
|
|
K | n |
|
|
K | n | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
PAmtR | 1.2 | 75 | 1.4 | 1.6 | 0.3 ± 0.1 | 80 ± 48 | 1.0 ± 0.7 | 1.7 ± 0.2 | ||||
PLitR | 1.4 | 85 | 1.0 | 1.7 | 1.9 ± 1.2 | 53 ± 36 | 2.7 ± 0.5 | 1.4 ± 0.2 | ||||
PBM3R1 | 0.1 | 10 | 2.9 | 2.9 | 0.0 ± 0.0 | 12 ± 8 | 1.6 ± 0.6 | 2.9 ± 0.2 | ||||
PSrpR | 0.1 | 41 | 1.2 | 2.8 | 0.3 ± 0.2 | 16 ± 2 | 3.0 ± 1.6 | 2.5 ± 0.2 | ||||
PPhlF | 0.4 | 80 | 2.5 | 3.9 | 0.7 ± 0.1 | 337 ± 2 | 8.9 ± 1.9 | 3.6 ± 0.2 |
Previously reported values (in au/s) based on a fluorescent reporter (Nielsen et al, 2016), converted to au/s as described in the text. The units of δJ off, δJ on, , , and K are au/s.
The units of δJ off, δJ on, , , and K are au/s. Average and standard deviations are calculated from three replicates measured on different days.