Table 3.
PCR assays for the detection of Brucella antigen in dogs.
| Primers directed to | Detection: Genus or Species | Sample | Sensitivity | Specificity |
|---|---|---|---|---|
| 16S-23S rDNA Interspace region (44) | Brucella genus | Whole blood | 100% | 100% |
| 16S-23S rDNA Interspace region (45)a | Brucella genus | Vaginal swabs | Not available | Not available |
| 16S-23S rDNA Interspace region (46)a | Brucella genus | Semen | Not available | Not available |
| 16S rRNA sequence (47) | Brucella genus | Whole blood | 100% | 100% |
| 16S-23S rRNA Interspace region (48) | Brucella genus | Inguinal lymph node | 100% | 100% |
| 16S-23S rRNA Interspace region (49) | Brucella genus | Whole blood Serum | Whole blood: 97.14% Serum: 25.71% |
Whole blood: 100% Serum: 100% |
| Intergenic spacer IS711 fragment (23)b | Brucella genus | Males: preputial swab, semen, or urine Female: vaginal swab |
Not available | Not available |
| Gene fragment on chromosome 1 (23)b | Brucella canis species-specific | As immediately above | Not available | Not available |
| B. canis outer membrane protein 25 DNA quantitative PCR (50) | Brucella canis species-specific | Vaginal swabs Whole blood |
Vaginal swabs: 92.31% Whole blood: 16.67% |
Vaginal swabs: 51.92% Whole blood: 100% |
| BCAN_B0548-0549 region in chromosome 2 of Brucella canis (51)c | Brucella canis species-specific | Whole blood Buffy coats |
Not available | Not available |
a
PCR on vaginal swabs and semen in these studies correlated with blood PCR, and blood culture as assessed by a Kappa co-efficient and the McNemar test.
b
Both Brucella genus-based and Brucella canis specific PCRs used in Swedish outbreak investigation.
c
Brucella canis inoculated samples; PCR on buffy coats separated from whole blood was approximately 100 times more sensitive than from whole blood.