Table 1.
Polymerase chain reaction (PCR) amplification primers used for amplification of Babesia odocoilei from cervid tissue samples.
| Amplified fragment | Product size (bp) | Primer names | Primer sequences (5′–3′) |
|---|---|---|---|
| Ribosomal 18S rDNAa | 1687 | Medlin A (F)b | AACCTGGTTGATCCTGCCAGT |
| Piro_18S_1688_Rc | CGACTTCTCCTTCCTTTAAGTGATAAG | ||
| Ribosomal 18s rDNAd | 681 | Piro_144_S | ACCGTGCTAATTGTAGGGCTAATACA |
| BCOMMON2R | TGCTTTCGCAGTAGTTCGTC |
a
PCR performed by the Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
b
Primer A of Medlin et al (20) less polylinker region.
c
Equivalent to primer BN1700 of Ramos et al (21).
d
PCR performed by the Vector Borne Disease Laboratory, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA, using primers described by Schoelkopf et al (7).