Figure 1.

Effect of dynein inhibition on the porcine oocyte maturation. (A) Representative images of oocyte meiotic progression in control and Ciliobrevin D-treated oocytes. Cumulus cell expansion of COCs and polar body extrusion of DOs were imaged by confocal microscope. Scale bar, 150 μm (a, b); 80 μm (c, d); 20 μm (e, f). (B) The rates of polar body extrusion were recorded in control and different concentrations of Ciliobrevin D groups (5 μM, 50 μM and 100 μM) after culture for 44 h in vitro. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a p < 0.05 level of significance.