Figure 2.

Effect of dynein inhibition on the spindle assembly and chromosome alignment in porcine oocytes. (A) Representative images of spindle morphologies and chromosome alignment in control and Ciliobrevin D-treated oocytes. Oocytes were immnunostained with anti-α-tubulin-FITC antibody to visualize the spindles and counterstained with Hoechst to visualize the chromosomes. Scale bar, 5 μm. Arrows indicate the abnormalities. (B) The rate of aberrant spindles was recorded in control and Ciliobrevin D-treated oocytes. (C) The rate of misaligned chromosomes was recorded in control and Ciliobrevin D-treated oocytes. Data in (B) and (C) were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a p < 0.05 level of significance.