Figure 3.

Effect of dynein inhibition on the microtubule stability in porcine oocytes. (A) Representative images of acetylated α-tubulin in control and Ciliobrevin D-treated oocytes. Oocytes were immnunostained with anti-acetyl-α-tubulin (Lys-40) antibody to assess the acetylation level of α-tubulin. Scale bar, 5 μm. (B) Quantitative analysis of the fluorescence intensity of acetylated α-tubulin in control and Ciliobrevin D-treated oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. Asterisk denotes statistical difference at a p < 0.05 level of significance.