Figure 4.

Let-7i/BAG-1 axis mediated the cisplatin resistance of lncRNA XIST on LAD. (A) MTT assays and (B) colony formation assay revealed that knockdown of XIST decreased cell proliferation and increase apoptosis, while anti-let-7i treatment rescued this effect; (C) Schematic representation of the predicted binding sites between let-7i and BAG-1, and the mutagenesis design for the reporter assays; (D) The luciferase assay showed that cells transfected with let-7i had less luciferase activity than those transfected with miR-ctrl. (E) The WB assay showed that cells transfected with let-7i repressed BAG-1 expression; (F) BAG-1 expression in A549 and A549/DDP cells; (G) MTT assays and (H) colony formation assay revealed that knockdown of BAG-1 decreased cell proliferation and increase apoptosis in XIST overexpression A549 cells. *P < 0.05, **P < 0.01.