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. 2017 Sep 29;16(21):2046–2057. doi: 10.1080/15384101.2017.1284713

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© 2017 Taylor & Francis

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Figure 1. — Localization of vinexin at the midbody during mitosis. (A) Live imaging of asynchronized HeLa cells overexpressing EGFP-vinexin β (Vxnβ) and RFP-Histone 2B (H2B). Midbody dense structure formed during cytokinesis observed by differential interference contrast (DIC) microscopy. Arrows and arrowhead mark midbody and focal adhesion, respectively. (B) After nocodazole release, endogenous vinexin was distributed in the cytosol from metaphase to anaphase but enriched at the midbody during cytokinesis. The mitotic stages were defined by the patterns of α-tubulin and Hoechst staining. (B′) A 3D-image reconstructed from confocal z stacks to show the entire midbody structure. (C) Isolated midbodies from Chinese hamster ovary (CHO) cells immunostained for vinexin and α-tubulin. Scales, 10 µm.