Figure 5.

Midbody localization of rhotekin depends on vinexin. (A) Immunostaining of rhotekin (RTKN) and α-tubulin at the midbody of HeLa cells. (B) HeLa cells expressing myc-tagged rhotekin (myc-RTKN) were immunostained with vinexin and myc antibodies. Midbody dense structure observed by DIC microscopy. (C-E) siCtrl, siVxn and siRTKN-2 cells released from mitotic arrest were immunolabeled with the denoted antibodies. The fluorescence intensity of rhotekin, vinexin or RhoA-GTP at the midbody of HeLa cells was quantified from 20 cells per group. Data are mean ± SEM. ** P < 0.01 by Student t test.