Figure 5.

G0S2 overexpression increases sensitivity of breast cancer cells to pharmacological inhibitors targeting the PI3K/mTOR pathway. BT474 and MCF7 cells stably expressing V5-tagged G0S2 or empty-vector control were treated with rapamycin, everolimus or BEZ235 for 7 to 10 days and assessed for changes in cell proliferation as compared to vehicles. Experiments were repeated three times using independently generated stable cell lines, and similar results were obtained. Data points are biological triplicates. Error bars, SD. *, P < 0.05; **, P < 0.01; ***, P < 0.005. Similar results were seen with engineered T47D cells (Figure S2).