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. 2017 Sep 28;16(21):2058–2072. doi: 10.1080/15384101.2017.1338221

Figure 5.

Figure 5.

Arsenic inhibits the expression of the ERα protein and mRNA across the cell cycle starting at 10 h but overexpression of ERα does not restore E2F1 expression. (A) Western blot analysis of ERα protein expression at times after treatment of quiescent cells with 5 nM E2 ± 5 µM iAs. GAPDH is a loading control. (B) The expression of ERα mRNA from the same experiment as in (A) measured by qRT-PCR. (n = 3; Error bars = SEM; *p-Value < 0.001 at 10–38 h). Experiment was repeated 3 times. (C) Transient transfection with 0.25 µg or 0.5 µg ERα in cells treated with 5nM E2 ± 5 µM iAs for 14 h and analyzed for expression of E2F1 mRNA by qRT-PCR. (n = 3; Error bars = SEM; *p-Value < 0.001 at 10–38 h). Below is shown the Western blot analysis of the expression of ERα protein in the cells incubated with an antibody to ERα with GAPDH as a loading control.