Figure 6.

Wnt1-Cre mediated haploinsufficiency of Eif4a3 disrupts Meckel’s cartilage development and causes premature clavicle ossification. (A, B) Whole mount E14.5 Eif4a3^l°^x/+ (A) and Wnt1-Cre; Eif4a3^l°^x/+ embryos (B) with number of embryos analyzed indicated. Skeletal preparations of E14.5 Eif4a3^l°^x/+(C, E) and Wnt1-Cre; Eif4a3^l°^x/+ embryos (D, F) showing low magnification and higher magnification images. Note cartilaginous and well-formed Meckel’s cartilage (blue staining, white circle) in control but underdeveloped in mutants (C–F). Note controls lack clavicle ossification (purple, white arrows) which is present in mutant clavicles (C–F). (G) Graph depicting quantification of fraction of embryos with ossified clavicles. Scales bars, (A, B) 5 mm, (C–F) 1 mm.