REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
AAV5.CaMKIIα.GCaMP6m	Grewe et al., 2017	N/A
AAV5. EF1α. Flex. mCherry	Penn Vector Core	N/A
Chemicals, Peptides, and Recombinant Proteins
L-368,899 hydrochloride	TOCRIS	26-411-0
Critical Commercial Assays
RNAscope Fluorescent Multiplex Reagent Kit	Advanced Cell Diagnostics	320850
RNAscope probe Mm-Slc32a1	Advanced Cell Diagnostics	319191
RNAscope Probe Mm-Slc17a6-C2	Advanced Cell Diagnostics	319171-C2
RNAscope Probe Mm-Oxtr-C3	Advanced Cell Diagnostics	402651-C3
Experimental Models: Organisms/Strains
Mouse: wild type C57BL/6J	Jackson Laboratory	JAX:000664
Mouse: Vgat-ires-Cre	Watabe-Uchida et al., 2012	N/A
Mouse: Vglut2-ires-Cre	Krashes et al., 2014	N/A
Software and Algorithms
ImageJ	NIH	https://imagej.nih.gov/ij/
Cellprofiler	Broad Institute	http://cellprofiler.org/
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
Ca2+ signal acquision	nVista	https://www.inscopix.com/
Cellsort MATLAB algorithm for extracting cellular Ca2+ signals	Ziv et al., 2013	N/A
Registration of cell identities across imaging sessions	Grewe et al., 2017	N/A
Fast nonnegative deconvolution	Vogelstein et al., 2010	N/A
Other
0.85-mm custom GRIN lens	Grintech	http://www.grintech.de/
Miniatureized microscope camera	Inscopix	https://www.inscopix.com/