Fig. 8.
Anti-PD-1 invigorates the site I-specific TCD8 response following immunization with DCs simultaneously displaying sites I and IV. B6 mice were injected i.v. with BMDCs co-pulsed with synthetic peptides corresponding to sites I and IV (A) or with mixed BMDC populations separately pulsed with each peptide alone (B) before they were treated with anti-PD-1 or isotype control (n=4 per cohort). Seven days later, mice were sacrificed, and splenic site I-specific TCD8 were enumerated by ICS for IFN-γ and by tetramer staining in parallel. * and ** denote p<0.05 and p<0.01, respectively, by Student’s t-test (A). Error bars represent SEM (A–B). NS: non-significant (B)