Fig. 9.
PD-1 blockade enhances site I-specific TCD8 survival but not their proliferative capacity. (A) Nine days after priming with C57SV cells, the frequencies of Ki-67+ cells and the MFI of Ki-67 expression within splenic sites I- and IV-specific TCD8 populations were determined in anti-PD-1-treated and control mice. Representative contour plots and mean ± SEM values are shown for 4 mice per group. (B) FLICA fluorescence as an active indicator of intracellular caspase levels was also assessed by flow cytometry. Representative histograms are illustrated, and bar graphs depict the results obtained from 4 mice per group (B). Error bars (A–B) represent SEM, and ** denotes p<0.01 by Student’s t-test (B).