Figure 2.

Chronic incubation with NED-19 results in an accumulation of lysosomal membrane, but does not impair the endocytic pathway. (A) NED-19 treatment resulted in an increase in the amount of Lysotracker-labelled lysosomes (green) compared to vehicle control (Con). In both control and NED-19 treated cells, FM 4–64 (red) loading in recycling endosomes co-localized (yellow) with Lysotracker after 16–20 hours, suggesting normal function in the endocytic pathway (scale bar = 5 µm). (Bi) Quantification of co-localization of FM 4–64 and Lysotracker staining. (Bii) Quantification of lysosomal area. (C). EM images. Multi-lamellar vesicular bodies were observed in the cell cytosol of NED-19 treated cells. (D) Quantification of lysosome diameter from EM images. (Left) Scale bar = 5 µm. Image in the white region is magnified 10x on the right. (Right) Scale bar = 0.5 µm. Error bars represent S.E.M. (n = 6–10 cells/condition). Non-significant group comparisons are denoted by n.s.