Table E2.
| Gene | Protein Change | DNA Change | Chr | Position | Forward Primer | Reverse Primer |
|---|---|---|---|---|---|---|
| JAK2 | p.V617F | c.1849G>T | 9 | 5073770 | CAAGCAGCA AGTATGAT GAGCAAGC |
CTGACACC TAGCTGTG ATCCTGAA |
| CPN2 | p.V292F | c.874G>T | 3 | 194062558 | TGGGAGGT GGGTAATG GCATTGTA |
TCCATCTT TGCCTCCC TGGGTAAT |
| HADHA | p.R291Q | c.872G>A | 2 | 26437358 | TGGTCCAG AATGGCAA TAAGGAGGA |
ACAGAATT GACAGCGTA TGCCATGA |
| CHEK2 | p.E231D | c.693A>T | 22 | 29120993 | CACGCCCA GCAACTTA CTCATCTT |
GAAGATCA CAGTGGCAA TGGAACC |
| SETD1A | p.Y382C | c.1145A>G | 16 | 30976208 | CTCCTCAT TGTCCTCG TCCTCCTC |
AGGAGGTG TAAGAAGGT GGGAAGC |
Mutation locations and primer details used for capillary sequencing. p., Protein; c., cDNA; Chr, chromosome. Genomic coordinates are from the hg19 reference genome.