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. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: J Am Chem Soc. 2017 Mar 9;139(11):3946–3949. doi: 10.1021/jacs.6b13271

Figure 2.

Figure 2

Generation of AWKX-MESNa thioesters. A. Proposed mechanism of thioester formation from peptide-MEGA. B-D. C18 RP-HPLC time-course of thioester formation for AWKG-MEGA (B), AWKA-MEGA (C) and AWKL-MEGA (D) peptides. Buffer: 200–400 mM MESNa, 100 mM NaH2PO4, 25–50 mM TCEP. RP-HPLC: 10–60% CH3CN in water, 30 min gradient.