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. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: J Am Chem Soc. 2017 Mar 9;139(11):3946–3949. doi: 10.1021/jacs.6b13271

Table 2.

Optimized thioesterification conditions for AWKX-MEGA peptides.

Entry X Temperature (°C) pH Reaction Time (h) Yield (%)a
1 L 70 4.5 48 67.8
2 K 70 4.5 48 67.5
3 F 70 4.5 48 65.8
4 G 37 5.6 72 60.5
5 D-A 70 5.6 24 57.1
6 Q 50 4.5 72 48.8
7 A 70 5.6 24 45.2
8 T 70 4.5 48 43.1
9 S 50 4.5 48 42.8
10 R 70 5.6 72 41.1
11 D-C 50 4.5 48 38.3
12 D 50 5.6 8 32.5
13 C 50 4.5 48 32.5
14 V 70 5.6 72 26.3
a

Based on RP-HPLC peak integration at 280 nm.