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. Author manuscript; available in PMC: 2018 Dec 6.
Published in final edited form as: Neuron. 2017 Nov 2;96(5):1041–1054.e5. doi: 10.1016/j.neuron.2017.10.010

Figure 4. The interaction between DISC1 and Ndel1 regulates the cell cycle progression of RGCs in developing mouse cortex in vivo.

Figure 4

(A and B) Expression of DISC1 765–835 (GFP-DISC1), but not GFP-DISC1 765–835 L822Q (GFP-DISC1-L822Q), leads to delayed cell cycle progression and results in increased mitotic index and decreased cell cycle exit index of RGCs. Shown on left are sample confocal images of brain sections immunostained with GFP, pH3, EdU, and DAPI (A) or GFP, Ki67, EdU, and DAPI (B). White arrows indicate GFP+EdU+pH3+ cells (A) and GFP+EdU+Ki67- cells (B). Quantifications are shown on the right for each panel. Scale bars: 20 μm. Numbers associated with each bar graph refer to the total number of brain sections analyzed under each condition. Values represent mean ± SEM (*P < 0.05, One-way ANOVA).

(C) Shown on the left are histograms for FACS analysis of dissociated embryonic cortical cells after electroporation. Bar graph depicts the percentage of GFP positive cells in G0/G1. Number associated with each bar graph refers to the number of replicates of flow cytometry under each condition. Values represent mean ± SEM (**P < 0.01, One-way ANOVA). See also Figure S5.