Table 2. Primers used in this study.

#	Name	Sequence
1	bnl-lexA gRNA fwd	TATATAGGAAAGATATCCGGGTGAACTTCgTGTATCTGCGATGCCCCTCAGTTTTAGAGCTAGAAATAGCAAG
2	bnl-lexA gRNA rev	ATTTTAACTTGCTATTTCTAGCTCTAAAACTCCCGCAATATCTGAAGGATcGACGTTAAATTGAAAATAGGTC
3	bnl N-F_pUC19	AATTCGAGCTCGGTACtgtggtctttgaggctggaac
4	bnl-lexA-N-R	tCCGcaagtCagtAGgctgccgcgtccttcgccggaGCCCGCAGATACAAGGCCCC
5	lexA-F	CTactGacttgCGGaGAtGTcGAaGAGAACCCtGGCCCtATGCCACCCAAGAAGAAGC
6	lexA-R	CTAAACGAGTTTTTAAGCAAACTCACTC
7	bnl lexA-C Fwd	TAAAAACTCGTTTAGACGGGATGGCGTTGTCAAC
8	bnl C-R_pUC19	GCCAAGCTTGCATGCCtcgcataattgccgcctgg
9	bnl-lexA scr fwd1	GTGGCGCACGCCCAATAAAC
10	bnl-lexA scr rev1	GATCCCAGCCAATCTCCGTTG
11	bnl-lexA scr fwd2	CAACGGAGATTGGCTGGGATC
12	bnl-lexA scr rev2	CTGGCCAACTGTAGGGAAGTC
13	ends-in check rev3	GCAATGTTATGCAATGCGTTGAC
14	bnl-lexA seq fwd3	CACTTGTCGCCCATATTGATACAATTG
15	lexA primer 5F	GATATGGATTTCTCCGCTTTGCTG
16	FGF domain R2	CCATGCAGAGATACAGGCAAGTG

Primer #1,2: were used for gRNA cloning; nucleotides underlined anneal to U6 promoter or gRNA core on pCFD4 vector, the lowercase g or c was added to aid transcription by the U6 promoter. Primer # 3, 4, 5, 6, 7, 8: for replacement donor construction, nucleotides in capital from # 3 or #8 overlap with pUC19 vector for Gibson Assembly, nucleotides underlined in # 4 or #5 were sequence overhang for T2A peptide addition. Primer # 9-14: were used for CRISPR screening and sequencing and were shown in Fig 2 as fwd1-3 and rev1-3. Primer # 15, 16: were used for RT-PCR analyses and were shown in Figure 2 as RT-f and RT-r.