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. 2017 Oct 10;36(22):3356–3371. doi: 10.15252/embj.201796797

Figure 5. MAM participates in the regulation of sphingolipid metabolism.

Figure 5

  1. Distribution of SMase activity in the indicated subcellular compartments in WT and PS‐DKO MEFs. Note the overall increase in nSMase levels in the PS‐DKO cells (average of n = 5 independent experiments ± SD). *P < 0.05. Analysis by unpaired t‐test.
  2. Western blot of the indicated fractions from WT and PS‐DKO MEFs (probed with antibodies against the indicated markers) to detect the levels of nSMase protein in the indicated compartments (30 μg of protein per lane). TH, total homogenate; CM, crude membrane fraction.
  3. Sphingolipid levels in mitochondrial membranes extracted from the indicated cells after addition of fluorescent sphingomyelin (left, detection in TLC plates; right, quantitation) (average of n = 5 independent experiments ± SD). *P < 0.05 vs. WT levels. Analysis by unpaired t‐test.