Fig 2.

Diagrammatic representation of the transcription factor binding sites in the MCAK promoter-reporter constructs (A), comparison of the promoter activities among pGL2-basic, pGL2-530, pGL2-530/mutated p53-motif, and pGL2-320 constructs in HCT116 (p53^+/+) cells (B), and effect of ectopically expressed p53 on the promoter activities of pGL2-530, pGL2-530/mutated p53-motif, and pGL2-320 constructs in p53-deficient HCT116 cells (C). The cells were transiently transfected or cotransfected with individual the promoter-reporter constructs with wild-type p53-expression vector (pCMV-Neo/p53) or empty-vector (pCMV-Neo). The pSV-β-galactosidase control vector was cotransfected in each experiment to correct for variations in transfection efficiency. The luciferase and β-galactosidase activities were measured as described in Materials and methods. Each value is expressed as the mean ± SD (n = 3). *Statistical significance was defined as p<0.05 compared to the control.