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. 2017 Dec 15;12(12):e0189698. doi: 10.1371/journal.pone.0189698

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© 2017 Jun et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Nucleotide sequence and putative transcription factor binding sites (A), and mutational analysis of the two p53-REs (p53-RE1 at −173/−166 and p53-RE2 at −245/−238) and two Sp1 binding motifs (GC1 at −93/−84 and GC2 at −119/−110) (B) in the human MCAK promoter (pGL2-320-Luc). The nucleotide sequence of the MCAK promoter region from −266 to +54 was analyzed and the positions of the putative transcription factor binding site are underlined, and the transcription start site is designated as +1. The translation start codon ATG is shaded light gray. The individual site-directed mutations were introduced within the pGL2-320-Luc vector. Transient transfection with each mutation construct was performed in HCT116 (p53^+/+) and HCT116 (p53^−/−) cells, and then luciferase activity was determined. Each value is expressed as the mean ± SD (n = 3). *Statistical significance was defined as p<0.05 compared to the control.