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. 2017 Dec 15;12(12):e0189698. doi: 10.1371/journal.pone.0189698

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© 2017 Jun et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Differential effect of Sp1 and p53 on the MCAK core promoter (pGL2-320-Luc) activity (A), the pG5-5×(GC)-Luc activity (B), and pGL2-mdm2 promoter (pGL-mdm2-Luc) activity (C). One hundred nanograms of individual promoter-reporter constructs (pGL2-320-Luc, pG5-5×(GC)-Luc, and pGL2-mdm2-Luc) were cotransfected with pCAGGS-Sp1, pCAGGS-wt-p53, or pCAGGS control empty vector into HCT116 (p53^-/-) cells, and then the luciferase activity was determined. Each value is expressed as the mean ± SD (n = 3). *Statistical significance was defined as p<0.05 compared to the control (100 ng of pCAGGS). To analyze the expression level of MCAK, Sp1, and β-actin proteins in the cells after transient transfection, western blot analyses were performed as described in Materials and methods. A representative study is shown and two additional experiments yielded similar results.