Figure 3.

FAE-extracted RNA in downstream enzymatic applications. (A) Lack of degradation of FAE-extracted RNA after a 15-min incubation in DNase buffer at 37°C. RNA was prepared using one-step FAE extraction or additionally purified with phenol-chloroform. After the incubation, RNA was precipitated, separated on an agarose gel, and transferred to a membrane for hybridization. (B) Quantitative RT-PCR analysis of induction of heat shock genes relative to normal growth conditions (30°C log phase) using RNA isolated by the FAE and hot phenol extraction. Data represent mean and SD values in technical triplicates. (C) Quantitative RT-PCR analysis of gene expression in stationary phase relative to log phase cultures. Samples were analyzed in the same way as in panel B.