Table 1. Competition of Pol IIIcore and Pol IV.
Concentration (nM) | Polymerase exchange (%)* | Lag time (s)† | Lifetime (s)‡ | ||||
---|---|---|---|---|---|---|---|
Competition | Pol IIIcore | Pol IV | III→IV | IV →III | III + IV | III→IV | Pol IIIcore |
Pol IIIcore - Pol IV | 30 | 6 | 81 | 12 | 7 | 32.5 ± 4.6 | 16.3 ± 1.0 |
30 | 30 | 70 | 22 | 9 | 20.3 ± 3.5 | 15.7 ± 1.1 | |
30 | 150 | 63 | 20 | 15 | 5.9 ± 0.5 | 16.6 ± 1.7 | |
150 | 150 | 51 | 24 | 26 | 3.5 ± 0.2 | 16.0 ± 0.9 | |
τ-complex§ - Pol IV | 30# | 30 | 95 | 0 | 5 | 11.3 ± 1.3 | 14.8 ± 0.9 |
*Polymerase exchange observed on clamp-DNA showing the exchange from Pol IIIcore to Pol IV (III→IV), Pol IV to Pol IIIcore (IV→III), or co-localization of Pol IIIcore and Pol IV (III + IV).
†Time between Pol IIIcore release and Pol IV arrival.
‡Lifetime on clamp-DNA.
§τ-complex consists of τ clamp loader (τ3δ1δ'1) and three Pol IIIcore complexes (α, ε, θ).
#Concentration of Pol IIIcore.