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. 2017 Dec 6;6:e32177. doi: 10.7554/eLife.32177

Table 1. Competition of Pol IIIcore and Pol IV.

Concentration (nM) Polymerase exchange (%)* Lag time (s) Lifetime (s)
Competition Pol IIIcore Pol IV III→IV IV →III III + IV III→IV Pol IIIcore
Pol IIIcore - Pol IV 30 6 81 12 7 32.5 ± 4.6 16.3 ± 1.0
30 30 70 22 9 20.3 ± 3.5 15.7 ± 1.1
30 150 63 20 15 5.9 ± 0.5 16.6 ± 1.7
150 150 51 24 26 3.5 ± 0.2 16.0 ± 0.9
τ-complex§ - Pol IV 30# 30 95 0 5 11.3 ± 1.3 14.8 ± 0.9

*Polymerase exchange observed on clamp-DNA showing the exchange from Pol IIIcore to Pol IV (III→IV), Pol IV to Pol IIIcore (IV→III), or co-localization of Pol IIIcore and Pol IV (III + IV).

Time between Pol IIIcore release and Pol IV arrival.

Lifetime on clamp-DNA.

§τ-complex consists of τ clamp loader (τ3δ1δ'1) and three Pol IIIcore complexes (α, ε, θ).

#Concentration of Pol IIIcore.