Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| Genetic reagent (Mus musculus) |
BGLAP-Cre;Rosa26mT/mG | This paper | NA |
| Genetic reagent (M. musculus) |
Ptch1c/c;BGLAP-Cre | Mak et al. (2006) | PMID: 16936073; DOI: 10.1242/dev.02546 RRID:MGI:3687744 |
| Genetic reagent (M. musculus) |
Smoc/c;BGLAP-Cre | Mak et al. (2008a) | PMID: 18434416; DOI: 10.1242/dev.018044 RRID:MGI:5311416 |
| Genetic reagent (M. musculus) |
Ptch1LacZ/+ | This paper | NA |
| Recombinant DNA reagent | pGL3-Mkxp8K | This paper | NA |
| Recombinant DNA reagent | pGL3-Mkx4K | This paper | NA |
| Recombinant DNA reagent | pCMV5 SMAD2-HA | Addgene, USA | plasmid # 14930 |
| Recombinant DNA reagent | pCMV5B-Flag-SMAD3 | Addgene, USA | plasmid # 11742 |
| Antibody | Osteolcalcin/Bglap | Abcam, USA | ab93876, 5 ug/ml RRID: AB_10675660 |
| Antibody | Tppp3 | Abcam, USA | ab150998, 1:50 RRID: AB_2716739 |
| Antibody | Mkx | Lifespan Biosciences Inc., USA | LS-B8063, 1 ug/ml RRID: AB_2716740 |
| Antibody | Mkx | Abcam, USA | ab179597, 1 ug/ml RRID: AB_2716741 |
| Antibody | Gli1 | R&D, USA | MAB3324, 10 ug/ml RRID:AB_2111775 |
| Antibody | Gli2 | R&D, USA | AF3635, 5 ug/ml RRID:AB_2111902 |
| Antibody | pH3 | SantaCruz, USA | sc-8656-R, 1:200 RRID:AB_653256 |
| Antibody | Collagen I | Abcam, USA | ab292, 1:1000 RRID:AB_303415 |
| Antibody | TGF-b1 | Abcam, USA | ab64715, 1:500 RRID:AB_1144265 |
| Antibody | Smad3 | CST, USA | #9523, 1:1000 RRID:AB_2193182 |
| Antibody | p-Smad3 | CST, USA | #9520, 1:1000 RRID:AB_2193207 |
| Antibody | β-Actin | CST, USA | #8457, 1:10000 RRID:AB_10950489 |
| Commercial assay or kit | Apoptosis Fluorescein Detection Kit | Millipore, USA | s7111 |
| Commercial assay or kit | Hydroxyproline Assay Kit | Sigma-Aldrich, USA | MAK008 |
| Commercial assay or kit | Dual-luciferase reporter assay system | Promega, USA | E1980 |
| Chemical compound, drug | BMP2 | CST, USA | #4697 |
| Chemical compound, drug | TGF-β1 | Peprotech, USA | 100–21 |
| Chemical compound, drug | Purmorphamine | Millipore, USA | 540220 |
| Chemical compound, drug | SB431542 | Sigma-Aldrich, USA | S4317 |