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. 2017 May 23;8(60):101026–101041. doi: 10.18632/oncotarget.18197

Figure 3. let-7g suppresses NF-κB signaling leading to anti-apoptosis for macrophages.

Figure 3

(A) let-7g affected gene expression in the p53-signaling in oxLDL-treated macrophages. (B) Bioinformatic predicted let-7g's binding site in the 3′-UTR of the IKKα transcript. (C) let-7g dose-dependently knocked down luciferase activity in the cells carrying wild-type IKKα plasmid, but let-7g had no effect on luciferase activity in the cells carrying the mutant IKKα plasmid. (D) let-7g knocked down IKKα expression in macrophages. (E) Let-7g's effects on mRNA levels of MEKK1 and A20 in macrophages. (F, G) IKKα was detected by the immunofluorescent staining in apoE KO mice (n = 6 per group) under a HF diet for 6 or 12 weeks. LV-let7g treatment reduced the plaque size and IKKα expression in vivo, and LV-let7g-sponge had an opposite effects. Scale bars: 50 μm. Data are presented as mean ± SEM from 3 independent experiments. * : < 0.05; ** : < 0.01; *** : < 0.001.