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. 2017 Nov 8;8(60):101042–101056. doi: 10.18632/oncotarget.13205

Figure 5. MiR-6780a-5p downregulated E-cadherin expression by directly targeting its 3’-UTR.

Figure 5

qRT-PCR and western blotting for E-cadherin in 786-O and OS-RC-2 cells transfected with miR-6780a-5p mimics or inhibitor A-D. MiR-6780a-5p overexpression promoted migration and invasion in 786-O and OS-RC-2 cells E. miR-6780a-5p knockdown inhibited migration and invasion F. The two predicted sites of miR-6780a-5p binding to the E-cadherin 3’-UTR were detected using bioinformatics tools G & I. Mutated sites in the E-cadherin 3’-UTR are shown. The effect of miR-6780a-5p on luciferase activity induced by the psiCHECK™-2-CDH1-WT1, psiCHECK™-2-CDH1-mMUT1, psiCHECK™-2-CDH1-WT2 and psiCHECK™-2-CDH1-MUT2 reporter plasmids in 786-O cells H & J. Data are shown as means ± S.D. of three replicates (**P<0.01, ***P<0.001).