Table 1. The list of identified metabolites changed significantly between shSLC25A22 and pLKO cell (in order of descending fold change).
| No. | Compound name | m/z | RT/min | FC(s/p)a | P-value | MS Pattern | ∆ppm | Mode | Pathway |
|---|---|---|---|---|---|---|---|---|---|
| 1 | AICAR# | 339.0680 | 1.51 | 2.82 | 1.7E-06 | 110.0352, 97.0283, 127.0614 | 6.0 | +/− | Histidine metabolism, purine metabolism |
| 2 | N-Acetylglutamic acid | 190.0699 | 2.09 | 1.60 | 9.9E-06 | 190.0710, 172.0600, 130.0500 | 5.8 | +/− | Biosynthesis of amino acids |
| 3 | Sphingosine | 300.2879 | 13.38 | 1.54 | 2.4E-06 | 282.2783, 252.2679, 56.0497 | 5.9 | + | Sphingosine metabolism |
| 4 | L-Glutamine* | 147.0756 | 0.90 | 1.33 | 2.6E-05 | 147.0762, 130.0495, 84.0448 | 5.5 | + | Alanine, aspartate and glutamate metabolism |
| 5 | Pyrroline hydroxycarboxylic acid | 130.0492 | 0.91 | 1.31 | 9.2E-05 | 84.0447, 130.0493, 56.0505 | 5.2 | + | Arginine and proline metabolism |
| 6 | L-Glutamic acid* | 148.0595 | 0.92 | 1.29 | 1.1E-04 | 84.0446, 102.0547, 130.0497 | 6.3 | + | Alanine, aspartate and glutamate metabolism |
| 7 | Glutathione* | 308.0891 | 1.58 | 1.22 | 1.6E-02 | 308.0934, 233.0610, 162.0235 | 6.4 | +/− | Glutathione metabolism |
| 8 | Indole | 118.0644 | 6.05 | 1.21 | 6.9E-04 | 118.0648, 91.0540, 65.0386 | 6.0 | + | Phenylalanine, tyrosine and tryptophan metabolism |
| 9 | L-Threonine* | 120.0649 | 0.91 | 1.17 | 3.6E-02 | 56.0497, 74.0599, 102.0547 | 5.5 | + | Aminoacyl-tRNA biosynthesis, threonine metabolism |
| 10 | Glycerophosphocholine | 258.1086 | 0.91 | 1.15 | 1.4E-02 | 104.1067, 124.9995, 184.0729 | 5.8 | + | Glycerophopholipid metabolism |
| 11 | L-Methionine* | 150.0575 | 1.53 | 1.13 | 1.1E-02 | 150.0574, 133.0314, 104.0528 | 5.5 | + | Aminoacyl-tRNA biosynthesis, Cysteine and methionine metabolism |
| 12 | Pantothenic acid* | 220.1167 | 4.79 | 1.10 | 3.2E-02 | 90.0548, 220.1175, 202.1069 | 5.9 | +/− | Pantothenate and CoA biosynthesis |
| 13 | Creatine* | 132.0760 | 0.95 | 0.80 | 1.0E-10 | 132.0768, 90.0555, 87.0557 | 5.6 | +/− | Arginine and proline metabolism |
| 14 | Butyrylcarnitine | 232.1530 | 5.00 | 0.79 | 3.5E-03 | 232.1565, 173.0821, 85.0295 | 5.7 | + | Fatty acid oxidation |
| 15 | L-Asparagine* | 133.0599 | 0.90 | 0.79 | 9.8E-05 | 133.0602, 87.0551, 74.0242 | 6.8 | + | Alanine, aspartate and glutamate metabolism |
| 16 | L-Histidine* | 154.0612 | 0.86 | 0.78 | 1.2E-04 | 154.0613, 93.0446, 137.0346 | 6.5 | − | Aminoacyl-tRNA biosynthesis, histidine metabolism |
| 17 | L-gamma-glutamyl-L-leucine | 261.1430 | 5.56 | 0.78 | 8.2E-07 | 261.1450,198.1120, 132.1020 | 5.9 | + | Biosynthesis of amino acids |
| 18 | Xanthine | 151.0252 | 2.04 | 0.78 | 1.2E-02 | 151.0250, 108.0199 | 6.4 | − | Purine metabolism |
| 19 | ADP* | 426.0227 | 1.48 | 0.78 | 7.3E-03 | 426.0227, 158.9245, 78.9575 | 1.4 | − | Purine metabolism |
| 20 | L-Tyrosine* | 180.0659 | 4.28 | 0.77 | 5.0E-08 | 119.0490, 180.0659, 136.0757 | 3.9 | − | Aminoacyl-tRNA biosynthesis, tyrosine metabolism |
| 21 | L-Phenylalanine* | 164.0706 | 4.28 | 0.77 | 7.0E-08 | 147.0442, 164.0707, 72.0080 | 6.9 | − | Aminoacyl-tRNA biosynthesis, phenylalanine metabolism |
| 22 | N-Acetyl-L-methionine | 190.0538 | 5.68 | 0.77 | 1.7E-06 | 148.0428, 142.0499, 190.0537 | 2.7 | − | Protein biosynthesis |
| 23 | N1, N12-Diacetylspermine* | 287.2440 | 0.91 | 0.76 | 2.4E-02 | 100.0763, 171.1498 | 0.5 | Polyamine metabolism | |
| 24 | Gamma Glutamylglutamic acid | 277.1014 | 1.51 | 0.76 | 5.5E-03 | 84.0442, 148.0600, 130.0496 | 5.9 | + | Amino acids biosynthesis and metabolism |
| 25 | Uridine diphosphate glucose | 565.0485 | 1.75 | 0.75 | 3.6E-02 | 323.0290, 565.0483, 384.9848 | 1.4 | − | Nuclear sugar metabolism |
| 26 | N-Formyl-L-methionine | 176.0380 | 5.31 | 0.75 | 3.5E-11 | 98.0234, 128.0342, 176.0379 | 4.1 | − | Cysteine and methionine metabolism |
| 27 | L-Acetylcarnitine | 204.1219 | 1.51 | 0.75 | 8.9E-08 | 85.0282, 204.1227, 60.0809 | 5.5 | + | Fatty acid oxidation |
| 28 | L-Alanine* | 90.0545 | 0.90 | 0.75 | 4.2E-09 | 90.0544 | 5.3 | + | Alanine, aspartate and glutamate metabolism |
| 29 | Oxidized glutathione* | 613.1558 | 2.43 | 0.74 | 5.0E-02 | 613.1592, 538.1252, 484.1162 | 5.7 | +/− | Glutathione metabolism |
| 30 | N-Acetyl-L-aspartic acid | 174.0399 | 1.01 | 0.71 | 5.7E-13 | 88.0390, 130.0499, 58.0282 | 5.1 | − | Alanine, aspartate and glutamate metabolism |
| 31 | Gamma-Glutamyltyrosine | 311.1219 | 4.76 | 0.69 | 8.5E-08 | 311.1240, 248.0920, 182.0810 | 6.0 | + | Tyrosine metabolism |
| 32 | Uridine diphosphate-N-acetylglucosamine | 606.0754 | 2.67 | 0.69 | 4.7E-04 | 606.0750, 384.9849, 282.0388 | 1.8 | − | Nucleotide biosynthesis |
| 33 | L-Aspartic acid* | 132.0291 | 0.90 | 0.55 | 2.4E-08 | 132.0291, 115.0025, 88.0399 | 8.7 | − | Alanine, aspartate and glutamate metabolism |
| 34 | Taurine | 126.0213 | 0.91 | 0.49 | 1.9E-06 | 126.0214, 108.0109 | 5.4 | +/− | Taurine and hypotaurine metabolism |
| 35 | 3-Sulfinoalanine | 152.0013 | 0.92 | 0.48 | 1.9E-03 | 88.0390, 152.0017 | 6.7 | − | Taurine and hypotaurine metabolism |
Notes:
aFC (s/p) represented fold change between shSLC25A22 and pLKO cells.
bAAs and derivatives represented that amino acids and their derivatives.
*Represented the metabolite was identified by authentic standard and database, while the unmarked metabolite was identified by database.
#AICAR, 1-(5’-Phosphoribosyl)-5-amino-4-imidazolecarboxamide.